Composite
Part:BBa_K2717007:Design
Designed by: Chenyu Liu Group: iGEM18_BNU-China (2018-10-08)
RBS2000-gdh-v5 tag-his tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1569
Illegal AgeI site found at 2461 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2443
Design Notes
NONE
Source
The glucose dehydrogenase is isolated from the genome of the laboratory strain. V5 tag and His tag are obtained from our own lab. The sequence of rbs2000 is obtained from the Kit and we synthesize by ourselves.