Composite

Part:BBa_K2717007:Design

Designed by: Chenyu Liu   Group: iGEM18_BNU-China   (2018-10-08)


RBS2000-gdh-v5 tag-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1569
    Illegal AgeI site found at 2461
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2443


Design Notes

NONE

Source

The glucose dehydrogenase is isolated from the genome of the laboratory strain. V5 tag and His tag are obtained from our own lab. The sequence of rbs2000 is obtained from the Kit and we synthesize by ourselves.

References